Enrichment of mutants lacking the phosphoenolpyruvate-dependent phosphotransferase system of Vibrio parahaemolyticus by screening with methyl-alpha-D-glucoside.
نویسندگان
چکیده
Screening with methyl-alpha-d-glucoside was an efficient procedure for enrichment of mutants lacking the glucose transport system and of the pleiotropic mutants lacking the phosphoenolpyruvate-dependent phosphotransferase system in Vibrio parahaemolyticus.
منابع مشابه
The role of the phosphoenolpyruvate phosphotransferase system in the transport of N-acetyl-D-glucosamine by Escherichia coli.
The properties of an N-acetyl-d-glucosamine-transport system have been studied by following the intracellular accumulation of methyl 2-acetamido-2-deoxy-alpha-d-[1-(14)C]glucoside by Escherichia coli. The same analogue was used to assay phosphoenolpyruvate phosphotransferase activity of toluene-treated cells. Transport and phosphorylation are induced by growth on d-glucosamine or N-acetyl-d-glu...
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The nature of the interaction of glucose with toluene-treated cells of Escherichia coli leading to inhibition of adenylate cyclase was examined by the use of analogues. Those analogues with variations of the substituents about carbon atoms 1 or 2 (e.g. alpha-methylglucoside or 2-deoxyglucose) are inhibitory, and they are also substrates of the phosphoenolpyruvate-dependent sugar phosphotransfer...
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عنوان ژورنال:
- Journal of bacteriology
دوره 119 2 شماره
صفحات -
تاریخ انتشار 1974